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Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

8,635 Views

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08:10 min

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February 06, 2017

DOI:

10.3791/55038-v

DOI:
10.3791/55038-v

•

08:10 min
February 06, 2017

•

3 Views
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1Department of Microbiology and Immunology,Miller School of Medicine, University of Miami, 2Division of Infectious Diseases,Miller School of Medicine, University of Miami

Summary

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We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.

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EndocervicalGamma Delta T CellsFlow CytometryIsolationCharacterizationIntraepithelialImmune EventsCytobrushIMDM MediumCell StrainerFlow Cytometry AnalysisAntibodiesViability DyeCompensation ControlsMagnetic Bead IsolationTCR Gamma Delta

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Strbo, N., Romero, L., Alcaide, M., Fischl, M. Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells. J. Vis. Exp. (120), e55038, doi:10.3791/55038 (2017).

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