A subscription to JoVE is required to view this content. Sign in or start your free trial.
7,871 Views
•
11:14 min
•
January 06, 2017
DOI:
Summary
Automatically generated
We describe a framework incorporating straightforward biochemical and computational analysis to guide the characterization and crystallization of large coiled-coil domains. This framework can be adapted for globular proteins or extended to incorporate a variety of high-throughput techniques.
Read Article
Cite this Article
Zalewski, J. K., Heber, S., Mo, J. H., O'Conor, K., Hildebrand, J. D., VanDemark, A. P. Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins. J. Vis. Exp. (119), e54886, doi:10.3791/54886 (2017).
Copy