このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
7,871 Views
•
11:14 min
•
January 06, 2017
DOI:
概要
Automatically generated
We describe a framework incorporating straightforward biochemical and computational analysis to guide the characterization and crystallization of large coiled-coil domains. This framework can be adapted for globular proteins or extended to incorporate a variety of high-throughput techniques.
Read Article
Cite this Article
Zalewski, J. K., Heber, S., Mo, J. H., O'Conor, K., Hildebrand, J. D., VanDemark, A. P. Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins. J. Vis. Exp. (119), e54886, doi:10.3791/54886 (2017).
Copy