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Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
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Capitoli
- 00:05Titolo
- 00:48Express and Purify Proteins with Identified Domain Boundaries
- 02:38Characterize Protein Sample to Identify Those with Advantageous Properties
- 04:16Producing High Quality Crystals of the Coiled-coil SD2 Domain from Shroom
- 08:43Results: Evaluation of Expression, Purification, Characterization, and Crystallization of Candidate Coiled-coil Proteins
- 09:49Conclusion
We describe a framework incorporating straightforward biochemical and computational analysis to guide the characterization and crystallization of large coiled-coil domains. This framework can be adapted for globular proteins or extended to incorporate a variety of high-throughput techniques.
