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La combinazione di Wet and Dry tecniche di laboratorio per guidare la cristallizzazione di grandi dimensioni avvolto a spirale contenenti proteine
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January 06, 2017
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We describe a framework incorporating straightforward biochemical and computational analysis to guide the characterization and crystallization of large coiled-coil domains. This framework can be adapted for globular proteins or extended to incorporate a variety of high-throughput techniques.
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Zalewski, J. K., Heber, S., Mo, J. H., O'Conor, K., Hildebrand, J. D., VanDemark, A. P. Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins. J. Vis. Exp. (119), e54886, doi:10.3791/54886 (2017).
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