A subscription to JoVE is required to view this content. Sign in or start your free trial.
10,745 Views
•
12:06 min
•
February 01, 2017
DOI:
Summary
Automatically generated
A method to measure protein diffusion in membranes of primary immune cells using fluorescence correlation spectroscopy (FCS) is described. In this paper, the use of antibodies for fluorescent labeling is illustrated.
Explore More Videos
Molecular DiffusionPlasma MembranesPrimary LymphocytesFluorescence Correlation SpectroscopyImmunologyCell BiologyNatural Killer CellsMembrane Receptor DynamicsImmunotherapyCancerPrimary CellsCell LinesConfocal Laser Scanning MicroscopeFCS MeasurementsFluorophoresDyePoly-L-lysineCoverglass SlideObjective Lens
Read Article
Cite this Article
Staaf, E., Bagawath-Singh, S., Johansson, S. Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy. J. Vis. Exp. (120), e54756, doi:10.3791/54756 (2017).
Copy