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Eksperimentel infektion med<em> Listeria monocytogenes</em> Som en model til at studere Host Interferon-y Responses
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Immunology and Infection
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JoVE Journal Immunology and Infection
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Eksperimentel infektion med<em> Listeria monocytogenes</em> Som en model til at studere Host Interferon-y Responses

DOI:
10.3791/54554-v

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10:10 min

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November 16, 2016

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, , , ,
1Department of Immunology,University of Toronto, 2Toronto General Research Institute,University Health Network, 3Women’s College Research Institute

Chapters

  • 00:05Title
  • 00:54Glycerol Stock Long-term Storage Preparation
  • 02:18Experimental L. monocytogenes Inoculum Preparation and Infection
  • 03:44Peak Infection Spleen and Liver Bacterial Load Measurement
  • 06:16Effects of L. monocytogenes on CD4+ and CD8+ Cell IFN-gamma Responses
  • 07:43Results: Representative NK, NKT, and CD4 and CD8 T cell IFN-gamma Responses to L. monocytogenes
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver, hvordan man pode C57BL / 6J mus med EGD-stamme af Listeria monocytogenes (L. monocytogenes) og måle interferon-γ (IFN-γ) responser ved naturlig dræber (NK) celler, naturlige dræber T (NKT) celler, og adaptive T-lymfocytter efter infektion. Denne protokol beskrives også hvordan man fører overlevelse hos mus efter infektion med en modificeret LD50 dosis af patogenet.

Tags

Listeria MonocytogenesExperimental InfectionInterferon GammaC57 Black MiceBacterial LoadSurvivalBHI AgarBHI BrothOD600PBSCentrifugationDilution

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