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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

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30,571 Views

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12:50 min

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August 09, 2011

DOI:

10.3791/3076-v

DOI:
10.3791/3076-v

•

12:50 min
August 09, 2011

•

17 Views
, , ,
1St Vincent’s Institute, Department of Medicine,The University of Melbourne, 2Department of Microbiology and Immunology,The University of Melbourne

Summary

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Listeria monocytogenes is a model organism for studying immune responses and genetic susceptibility to intracellular bacteria in mice. This method enables one to measure bacterial load and generate single-cell suspensions of the liver and spleen from mice for FACS analysis to determine changes in immune cells due to Listeria infection.

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Wang, N., Strugnell, R., Wijburg, O., Brodnicki, T. Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes. J. Vis. Exp. (54), e3076, doi:10.3791/3076 (2011).

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