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10:10 min
November 16, 2016
DOI:
10.3791/54554-v
这个协议描述如何接种C57BL / 6J小鼠李斯特菌的EGD株(单增李斯特菌),并测量干扰素γ(IFN-γ)的反应由自然杀伤(NK)细胞,天然杀伤T(NKT)细胞,和自适应T淋巴细胞后感染。该协议还介绍了如何进行生存研究,小鼠感染后有修改的LD 50剂量的病原体。
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Ahn, J. J., Selvanantham, T., Zhang, M. A., Mallevaey, T., Dunn, S. E. Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses. J. Vis. Exp. (117), e54554, doi:10.3791/54554 (2016).
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