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Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
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JoVE 杂志 免疫与感染
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

13,321 Views

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10:10 min

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November 16, 2016

DOI:

10.3791/54554-v

DOI:
10.3791/54554-v

•

10:10 min
November 16, 2016

•

9 Views
, , , ,
1Department of Immunology,University of Toronto, 2Toronto General Research Institute,University Health Network, 3Women’s College Research Institute

Summary

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This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.

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Ahn, J. J., Selvanantham, T., Zhang, M. A., Mallevaey, T., Dunn, S. E. Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses. J. Vis. Exp. (117), e54554, doi:10.3791/54554 (2016).

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