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Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
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Immunology and Infection
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JoVE Journal Immunology and Infection
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

English

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10,164 Views

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05:17 min

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July 28, 2016

DOI:

10.3791/54484-v

DOI:
10.3791/54484-v

•

05:17 min
July 28, 2016

•

6 Views
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1Institute for Medical Physics and Biophysics, Medical Faculty,University of Leipzig, 2Fraunhofer Institute for Cell Therapy and Immunology (IZI) Leipzig

Summary

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This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.

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Cite this Article

Flemmig, J., Schwarz, P., Bäcker, I., Leichsenring, A., Lange, F., Arnhold, J. Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples. J. Vis. Exp. (113), e54484, doi:10.3791/54484 (2016).

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