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Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
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Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Capítulos
- 00:05Título
- 00:36Hypotonic Erythrocyte Lysis
- 02:20Halogenating Peroxidase Activity Staining
- 03:31Results: Leukocyte Enrichment and APF-derived Fluorescence in a Rat Blood Micro-sample
- 04:29Conclusion
This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.
