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Évaluation rapide et spécifique de l'halogénation peroxydase Activité dans les échantillons de sang enrichie en leucocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
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Évaluation rapide et spécifique de l'halogénation peroxydase Activité dans les échantillons de sang enrichie en leucocytes

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05:17 min

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July 28, 2016

DOI:

10.3791/54484-v

DOI:
10.3791/54484-v

•

05:17 min
July 28, 2016

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1Institute for Medical Physics and Biophysics, Medical Faculty,University of Leipzig, 2Fraunhofer Institute for Cell Therapy and Immunology (IZI) Leipzig

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Blood SampleLeukocyte EnrichmentHypotonic LysisPeroxidase ActivityAPF StainingHeme Peroxidase InhibitorHydrogen PeroxideCentrifugationHBSSCell Analysis

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Flemmig, J., Schwarz, P., Bäcker, I., Leichsenring, A., Lange, F., Arnhold, J. Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples. J. Vis. Exp. (113), e54484, doi:10.3791/54484 (2016).

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