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Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
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JoVE 杂志 免疫与感染
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

DOI:
10.3791/54484-v

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05:17 min

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July 28, 2016

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, , , , ,
1Institute for Medical Physics and Biophysics, Medical Faculty,University of Leipzig, 2Fraunhofer Institute for Cell Therapy and Immunology (IZI) Leipzig

Chapters

  • 00:05Title
  • 00:36Hypotonic Erythrocyte Lysis
  • 02:20Halogenating Peroxidase Activity Staining
  • 03:31Results: Leukocyte Enrichment and APF-derived Fluorescence in a Rat Blood Micro-sample
  • 04:29Conclusion

Summary

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This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.

Tags

Blood SampleLeukocyte EnrichmentHypotonic LysisPeroxidase ActivityAPF StainingHeme Peroxidase InhibitorHydrogen PeroxideCentrifugationHBSSCell Analysis

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