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La fusión absoluta y relativa de datos de PCR cuantitativa para cuantificar STAT3 variante de empalme transcripciones
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

La fusión absoluta y relativa de datos de PCR cuantitativa para cuantificar STAT3 variante de empalme transcripciones

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11:19 min

October 09, 2016

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11:19 min
October 09, 2016

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Summary

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Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.

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