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October 09, 2016
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Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.
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Turton, K. B., Esnault, S., Delain, L. P., Mosher, D. F. Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts. J. Vis. Exp. (116), e54473, doi:10.3791/54473 (2016).
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