Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Keren B. Turton; Stephane Esnault; Larissa P. Delain; Deane F. Mosher

doi:10.3791/54473

ジャーナル

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遺伝学

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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

JoVE Journal
遺伝学

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JoVE Journal 遺伝学

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

14,542 Views

•

11:19 min

•

October 09, 2016

DOI:

10.3791/54473-v

DOI:

10.3791/54473-v

•

11:19 min

October 09, 2016

•

5 Views

Keren B. Turton¹, Stephane Esnault², Larissa P. Delain², Deane F. Mosher^1,2

¹Department of Biomolecular Chemistry,University of Wisconsin-Madison, ²医学部,University of Wisconsin-Madison

概要

Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.

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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

概要

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