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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

DOI:
10.3791/54473-v

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11:19 min

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October 09, 2016

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, , ,
1Department of Biomolecular Chemistry,University of Wisconsin-Madison, 2医学部,University of Wisconsin-Madison

章

  • 00:05標題
  • 01:16Creating Plasmids as Template Standards
  • 02:20Analyzing Primer Specificity for Absolute qPCR
  • 05:49Performing Relative qPCR Assays
  • 06:57Analyzing Absolute qPCR Data for Unknown Samples
  • 08:27Results: Measurement of STAT3 Splice Variant Levels in Eosinophils Treated with Cytokines
  • 10:06Conclusion

概要

自動翻訳

自動翻訳

Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.

タグ

Quantitative PCRSTAT3 Splice VariantsEosinophilsAbsolute QuantificationRelative QuantificationPlasmid StandardsCytokine StimulationCDNASequencingPlasmid ConcentrationCopy NumberDilution SeriesNon-target ControlsTarget MixPrimer Pairs

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