Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Keren B. Turton; Stephane Esnault; Larissa P. Delain; Deane F. Mosher

doi:10.3791/54473

Journal

/

Genética

/

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

JoVE Journal
Genética

This content is Free Access.

JoVE Journal Genética

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

14,542 Views

•

11:19 min

•

October 09, 2016

DOI:

10.3791/54473-v

DOI:

10.3791/54473-v

•

11:19 min

October 09, 2016

•

5 Views

Keren B. Turton¹, Stephane Esnault², Larissa P. Delain², Deane F. Mosher^1,2

¹Department of Biomolecular Chemistry,University of Wisconsin-Madison, ²Department of Medicine,University of Wisconsin-Madison

Summary

Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.

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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

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