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在气动操纵液滴平台多核苷酸多态性的视觉比色法检测
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Genetics
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JoVE Journal Genetics
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
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在气动操纵液滴平台多核苷酸多态性的视觉比色法检测

DOI:
10.3791/54424-v

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10:01 min

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September 27, 2016

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, , , ,
1Department of Mechanical Engineering,National Taiwan University

Chapters

  • 00:05Title
  • 01:05Detecting Multi-nucleotide Polymorphisms by Hybridization-mediated Growth of Gold Nanoparticles
  • 03:36Fabrication of the Pneumatic Droplet Manipulation Platform
  • 05:58Detecting Multi-nucleotide Polymorphisms on the Pneumatic Droplet Manipulation Platform
  • 07:21Results: Colorimetric Detection of Multi-nucleotide Polymorphisms
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

这项工作提出了一个简单直观的方法来检测气动操纵液滴平台的多核苷酸多态性。与所提出的方法中，在整个实验，包括液滴操作和多核苷酸多态性检测，可以接近23°C不先进仪器的帮助下进行。

Tags

Multi-nucleotide PolymorphismsPneumatic Droplet ManipulationVisual Colorimetric DetectionGold NanoparticlesDNA DetectionGenetic VariationGenetic DiseasesProbe DNASodium ChlorideHydroxyl AmineChloroauric AcidNanoparticle Optical PropertiesDNA Fragment Mismatch

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