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Chapters
- 00:05Title
- 00:35Restriction Enzyme Treatment, Bisulfite Conversion, and Design of Bisulfite Sequencing Primers
- 01:54Bisulfite Converted PCR and Gel Extraction
- 04:16Bisulfite Sequencing Library Preparation
- 10:44Results: Accurate Quantification of mtDNA Methylation
- 11:48Conclusion
Here, we present a protocol to allow accurate quantification of mitochondrial DNA (mtDNA) methylation. In this protocol, we describe an enzymatic digestion of DNA with BamHI coupled with a bioinformatic analysis pipeline which can be used to avoid overestimation of mtDNA methylation levels caused by the secondary structure of mtDNA.
