Journal
/
Developmental Biology
/
Protocollo per la conversione diretta dei fibroblasti murini embrionali in trofoblasto cellule staminali
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
Please note that all translations are automatically generated. Click here for the English version.

Protocollo per la conversione diretta dei fibroblasti murini embrionali in trofoblasto cellule staminali

italiano

Automatically Generated

7,509 Views

•

08:57 min

•

July 25, 2016

DOI:

10.3791/54277-v

DOI:
10.3791/54277-v

•

08:57 min
July 25, 2016

•

2 Views
,
1Department of Developmental Pathology,Institute of Pathology, University of Bonn Medical School

Summary

Automatically generated

Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

Explore More Videos

Murine Embryonic FibroblastsTrophoblast Stem CellsInduced Trophoblast Stem CellsITSCsTransfectionLentiviral Vector293T CellsVirus ProductionCell CulturePoly-L-lysineChloroquineHEPES Buffered Saline

Read Article

Cite this Article

Kubaczka, C., Schorle, H. Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells. J. Vis. Exp. (113), e54277, doi:10.3791/54277 (2016).

Download .ris file

Copy

Share Video

.

Copy