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JoVE Journal Developmental Biology
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
 

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Article DOI: 10.3791/54277-v • 08:57 min • July 25th, 2016
July 25th, 2016 •
1, 1
1Department of Developmental Pathology, Institute of Pathology, University of Bonn Medical School

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Resumo
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Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

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Murine Embryonic Fibroblasts Trophoblast Stem Cells Induced Trophoblast Stem Cells Transgenic Expression Poly-L-lysine Solution 293 T Cells Transfection Medium Chloroquine Solution Lentiviral Vector HEPES Buffer
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