Journal
/
Biologie du développement
/
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

DOI:
10.3791/54277-v

•

08:57 min

•

July 25, 2016

•

,
1Department of Developmental Pathology,Institute of Pathology, University of Bonn Medical School

Chapitres

  • 00:05Titre
  • 00:40293T Cell Lentiviral Vector Production
  • 03:06Fibroblast to Induced Trophoblast Stem Cell (iTSC) Conversion
  • 05:10Individual iTSC Line Isolation (Transdifferentiation Day 21-28)
  • 07:02Results: Representative Morphological Changes During MEF to iTSC Conversion
  • 07:56Conclusion

Summary

Traduction automatique

Traduction automatique

Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

Tags

Murine Embryonic FibroblastsTrophoblast Stem CellsInduced Trophoblast Stem CellsITSCsTransfectionLentiviral Vector293T CellsVirus ProductionCell CulturePoly-L-lysineChloroquineHEPES Buffered Saline

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Single-cell Photoconversion in Living Intact Zebrafish

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Read Article