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Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

7,509 Views

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08:57 min

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July 25, 2016

DOI:

10.3791/54277-v

DOI:
10.3791/54277-v

•

08:57 min
July 25, 2016

•

2 Views
,
1Department of Developmental Pathology,Institute of Pathology, University of Bonn Medical School

Summary

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Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

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Cite this Article

Kubaczka, C., Schorle, H. Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells. J. Vis. Exp. (113), e54277, doi:10.3791/54277 (2016).

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