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Taze izole edilmiş insan Villöz sitotrofoblastlar ilgili siRNA transfeksiyonu ve EMSA analizi
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Genetics
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JoVE Journal Genetics
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
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Taze izole edilmiş insan Villöz sitotrofoblastlar ilgili siRNA transfeksiyonu ve EMSA analizi

Türkçe

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10,299 Views

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09:57 min

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September 20, 2016

DOI:

10.3791/53995-v

DOI:
10.3791/53995-v

•

09:57 min
September 20, 2016

•

15 Views
, , ,
1Department of Biological Sciences,University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics,McGill University, 3Department of Clinical Sciences,University of Montreal

Summary

Automatically generated

Bu protokol, etkili bir şekilde, taze izole edilmiş beşeri villöz sitotrofoblastlar siRNA transfekte gözeneklendirilmesinin kullanılarak bu hücrelerde DNA-protein kompleksleri tanımlanması için bir yöntem tarif eder. Transfekte edilen hücreler, Western Blot ile izlenebilir ve EMSA 4-günlük kültür döneminde analiz eder.

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Cite this Article

Lokossou, A. G., Toufaily, C., Vargas, A., Barbeau, B. siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts. J. Vis. Exp. (115), e53995, doi:10.3791/53995 (2016).

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