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siRNA Transfection e l'EMSA Analisi su appena isolate umani dei villi citotrofoblasti
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Genetics
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JoVE Journal Genetics
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
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siRNA Transfection e l'EMSA Analisi su appena isolate umani dei villi citotrofoblasti

italiano

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10,299 Views

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09:57 min

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September 20, 2016

DOI:

10.3791/53995-v

DOI:
10.3791/53995-v

•

09:57 min
September 20, 2016

•

15 Views
, , ,
1Department of Biological Sciences,University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics,McGill University, 3Department of Clinical Sciences,University of Montreal

Summary

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Questo protocollo descrive un metodo per trasfezione efficiente siRNA in appena isolate citotrofoblasti villi umani con microperforazione e identificando complessi DNA-proteina in queste cellule. cellule trasfettate possono essere monitorati da Western Blot e EMSA analisi durante il tempo della cultura di 4 giorni.

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Cite this Article

Lokossou, A. G., Toufaily, C., Vargas, A., Barbeau, B. siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts. J. Vis. Exp. (115), e53995, doi:10.3791/53995 (2016).

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