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As análises siRNA transfecção e EMSA sobre recentemente isolados das vilosidades citotrofoblastos Humanos
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Genetics
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JoVE Journal Genetics
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

As análises siRNA transfecção e EMSA sobre recentemente isolados das vilosidades citotrofoblastos Humanos

português

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10,268 Views

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09:57 min

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September 20, 2016

DOI:

10.3791/53995-v

DOI:
10.3791/53995-v

•

09:57 min
September 20, 2016

•

15 Views
, , ,
1Department of Biological Sciences,University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics,McGill University, 3Department of Clinical Sciences,University of Montreal

Summary

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Este protocolo descreve um método para eficazmente transfectar siARN em citotrofoblastos vilosidades humanos isolados de fresco utilizando formação de microporos e identificação de complexos de ADN-proteína nestas células. As células transfectadas pode ser monitorado por análises Western blot e EMSA durante o tempo de cultura de 4 dias.

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Cite this Article

Lokossou, A. G., Toufaily, C., Vargas, A., Barbeau, B. siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts. J. Vis. Exp. (115), e53995, doi:10.3791/53995 (2016).

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