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siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
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Genética
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JoVE Journal Genética
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

10,299 Views

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09:57 min

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September 20, 2016

DOI:

10.3791/53995-v

DOI:
10.3791/53995-v

•

09:57 min
September 20, 2016

•

15 Views
, , ,
1Department of Biological Sciences,University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics,McGill University, 3Department of Clinical Sciences,University of Montreal

Summary

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This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.

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Cite this Article

Lokossou, A. G., Toufaily, C., Vargas, A., Barbeau, B. siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts. J. Vis. Exp. (115), e53995, doi:10.3791/53995 (2016).

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