It is critical in neurobiology and neurovirology to have a reliable, replicable in vitro system that serves as a translational model for what occurs in vivo in human neurons. This protocol describes how to culture and differentiate SH-SY5Y human neuroblastoma cells into viable neurons for use in in vitro applications.
Shipley, M. M., Mangold, C. A., Szpara, M. L. Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line. J. Vis. Exp. (108), e53193, doi:10.3791/53193 (2016).