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JoVE Journal Developmental Biology
Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
 

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Article DOI: 10.3791/53193-v • 08:07 min • February 17th, 2016
February 17th, 2016 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University

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It is critical in neurobiology and neurovirology to have a reliable, replicable in vitro system that serves as a translational model for what occurs in vivo in human neurons. This protocol describes how to culture and differentiate SH-SY5Y human neuroblastoma cells into viable neurons for use in in vitro applications.

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Keywords: SH-SY5Y Human Neuroblastoma Cell Line Differentiation Neurons Infectious Disease Research Proteomic Genomic Tripsinization Triteration 1X PBS 0.05% Trypsin EDTA Basic Growth Media Retinoic Acid Differentiation Media
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