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Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
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JoVE Journal Gelişim Biyolojisi
Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

67,516 Views

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08:07 min

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February 17, 2016

DOI:

10.3791/53193-v

DOI:
10.3791/53193-v

•

08:07 min
February 17, 2016

•

158 Views
, ,
1Department of Biochemistry and Molecular Biology, The Huck Institutes of the Life Sciences,The Pennsylvania State University

Özet

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It is critical in neurobiology and neurovirology to have a reliable, replicable in vitro system that serves as a translational model for what occurs in vivo in human neurons. This protocol describes how to culture and differentiate SH-SY5Y human neuroblastoma cells into viable neurons for use in in vitro applications.

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SH-SY5YHuman NeuroblastomaCell LineDifferentiationNeuronsInfectious Disease ResearchProteomicGenomicTripsinizationTriteration1X PBS0.05% Trypsin EDTABasic Growth MediaRetinoic AcidDifferentiation Media

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Cite this Article

Shipley, M. M., Mangold, C. A., Szpara, M. L. Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line. J. Vis. Exp. (108), e53193, doi:10.3791/53193 (2016).

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