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Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
DOI:

08:07 min

February 17, 2016

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Capítulos

  • 00:05Título
  • 00:59Passage of SH-SY5Y Maintenance Cultures
  • 02:33Differentiation Procedures
  • 06:24Results: Images of Cells in the Intermediate Steps of the Differentiation Protocol
  • 07:28Conclusion

Summary

Traducción Automática

It is critical in neurobiology and neurovirology to have a reliable, replicable in vitro system that serves as a translational model for what occurs in vivo in human neurons. This protocol describes how to culture and differentiate SH-SY5Y human neuroblastoma cells into viable neurons for use in in vitro applications.

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