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Saç türetilmiş keratinositlerden Kullanarak Entegrasyon serbest İnsan uyarılmış pluripotent kök hücrelerin üretilmesi
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
DOI:

08:36 min

August 20, 2015

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Chapters

  • 00:05Title
  • 01:08Isolation of Keratinocytes From Plucked Hair
  • 03:16Maintenance and Passaging of Keratinocytes
  • 04:34Generation of hiPSCs from Keratinocytes using Episomal Vectors
  • 07:25Results: iPSCs Derived from Keratinocytes Express Pluripotent Markers
  • 08:11Conclusion

Summary

Automatic Translation

This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.

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