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Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
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Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
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August 20, 2015
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This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.
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Hung, S. S., Pébay, A., Wong, R. C. Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes. J. Vis. Exp. (102), e53174, doi:10.3791/53174 (2015).
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