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Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
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JoVE Journal Developmental Biology
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

English

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11,275 Views

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08:36 min

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August 20, 2015

DOI:

10.3791/53174-v

DOI:
10.3791/53174-v

•

08:36 min
August 20, 2015

•

12 Views
, ,
1Centre for Eye Research Australia & Department of Ophthalmology,University of Melbourne

Summary

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This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.

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Cite this Article

Hung, S. S., Pébay, A., Wong, R. C. Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes. J. Vis. Exp. (102), e53174, doi:10.3791/53174 (2015).

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