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Bacterial Artificial Chromosomes: uno strumento Genomica Funzionale per lo studio di positivo-strand virus RNA
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Chapters
- 00:05Title
- 01:18Summary of Procedures in Video Format
- 01:51High-purity Maxi-prep of Full-length SA14-14-2 BAC: Part 1
- 04:54High-purity Maxi-prep of Full-length SA14-14-2 BAC: Part 2
- 06:48Transcribe Synthetic RNAs In Vitro from a Linearized Full-length JEV BAC DNA
- 08:55Determine RNA Infectivity and Virus Yield
- 10:36Results: Specific Infectivity of the Synthetic RNAs Transcribed from a Full-length JEV BAC and the Recovery of Synthetic Virus
- 11:40Conclusion
Here, a protocol is presented for creating an infectious bacterial artificial chromosome containing a full-length cDNA of the positive-strand genomic RNA of Japanese encephalitis virus. This protocol can be used to construct a functional cDNA of other positive-strand RNA viruses, making it a powerful genomic tool for studying virus biology.
