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Interface 3D Engineered culturas neuronais para Micro-Arrays eletrodo: An Innovative<em> In Vitro</em> Modelo Experimental
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JoVE Journal Neuroscience
Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Interface 3D Engineered culturas neuronais para Micro-Arrays eletrodo: An Innovative<em> In Vitro</em> Modelo Experimental

DOI:
10.3791/53080-v

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09:47 min

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October 18, 2015

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, , , ,
1Department of Informatics, Bioengineering, Robotics and System Engineering (DIBRIS),University of Genova, 2Donders Institute for Brain, Cognition and Behaviour, Department of Cognitive Neuroscience,Radboud University Medical Center, 3Fondazione Istituto Italiano di Tecnologia (IIT)

Chapters

  • 00:05Title
  • 02:08Preparation of Materials and Supports
  • 04:18Dissection of Embryos, Tissue Dissociation, and Cell Plating
  • 06:323D Neuronal Network Construction
  • 07:28Results: Examples of 3D Networks Captured by Confocal Microscopy and by Electrophysiological Recordings
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.

Tags

Keywords: 3D Neuronal CulturesMicro-electrode ArraysIn Vitro Experimental Model2D Neural Networks3D NetworksHippocampal NeuronsCortical NeuronsGlass MicrobeadsElectrophysiological ActivityBursting ActivitySpiking ActivityIn Vivo Neural Networks

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