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JoVE Journal Neuroscience
Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays
 

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Article DOI: 10.3791/53080-v • 09:47 min • October 18th, 2015
October 18th, 2015 •
1, 1,2, 1, 3, 1
1Department of Informatics, Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, 2Donders Institute for Brain, Cognition and Behaviour, Department of Cognitive Neuroscience, Radboud University Medical Center, 3Fondazione Istituto Italiano di Tecnologia (IIT)

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In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.

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3D Engineered Neuronal Cultures Micro-Electrode Arrays In Vitro Experimental Model Large-scale Networks Dissociated Neurons Extracellular Micro-transducer Devices Neurophysiological Mechanisms Neuronal Cell Assemblies Electrophysiological Activity Bi-dimensional Neural Networks Three-dimensional Networks Hippocampal Networks Cortical Networks Planar Micro-Electrode Arrays (MEAs) Glass Microbeads Complex Interconnected Assemblies Morphological Organization Electrophysiological Patterns
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