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Zebrafisch Keratozyten Explantate auf Collective Zellmigration und Reepithelialisierung in kutane Wundheilung Studieren
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Developmental Biology
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JoVE Journal Developmental Biology
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
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Zebrafisch Keratozyten Explantate auf Collective Zellmigration und Reepithelialisierung in kutane Wundheilung Studieren

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9,788 Views

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09:35 min

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February 25, 2015

DOI:

10.3791/52489-v

DOI:
10.3791/52489-v

•

09:35 min
February 25, 2015

•

6 Views
, , , , ,
1Biomedical Sciences Program,Midwestern University, 2Department of Microbiology & Immunology,Midwestern University

Summary

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Zebrabärbling Keratozyten migrieren in Zellschichten aus Explantaten und stellen eine in vitro-Modell für die Untersuchung der Mechanismen der kollektiven Zellmigration im Rahmen der epithelialen Wundheilung. Diese Protokolle Detail ein effektiver Weg zur primären Explantatkulturen für den Einsatz in kollektive Zellmigrationsassays herzustellen.

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Cite this Article

Rapanan, J. L., Pascual, A. S., Uppalapati, C. K., Cooper, K. E., Leyva, K. J., Hull, E. E. Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing. J. Vis. Exp. (96), e52489, doi:10.3791/52489 (2015).

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