/
/
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
9,788 Views
•
09:35 min
•
February 25, 2015
DOI:
Summary
Automatically generated
Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.
Read Article
Cite this Article
Rapanan, J. L., Pascual, A. S., Uppalapati, C. K., Cooper, K. E., Leyva, K. J., Hull, E. E. Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing. J. Vis. Exp. (96), e52489, doi:10.3791/52489 (2015).
Copy