Journal
/
Biología del desarrollo
/
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

DOI:
10.3791/52489-v

•

09:35 min

•

February 25, 2015

•

, , , , ,
1Biomedical Sciences Program,Midwestern University, 2Department of Microbiology & Immunology,Midwestern University

Capítulos

  • 00:05Título
  • 01:55Preparation of Anesthesia, Equipment, and Media
  • 02:55Establish Explant Cultures
  • 04:40Brightfield and Video Microscopy
  • 05:20Fixation for Immunofluorescence Microscopy
  • 07:02Results: Cell Migration in Zebrafish Keratocytes
  • 08:40Conclusion

Summary

Traducción Automática

Traducción Automática

Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.

Tags

ZebrafishKeratocyteExplantCollective Cell MigrationReepithelializationCutaneous Wound HealingSingle Cell MigrationActin-rich LamellaeActin CableEpithelial To Mesenchymal TransitionGene ExpressionMutantTransgenicIn Vitro Model

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Read Article