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Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

DOI:
10.3791/52489-v

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09:35 min

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February 25, 2015

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, , , , ,
1Biomedical Sciences Program,Midwestern University, 2Department of Microbiology & Immunology,Midwestern University

Capitoli

  • 00:05Titolo
  • 01:55Preparation of Anesthesia, Equipment, and Media
  • 02:55Establish Explant Cultures
  • 04:40Brightfield and Video Microscopy
  • 05:20Fixation for Immunofluorescence Microscopy
  • 07:02Results: Cell Migration in Zebrafish Keratocytes
  • 08:40Conclusion

Summary

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Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.

Tags

ZebrafishKeratocyteExplantCollective Cell MigrationReepithelializationCutaneous Wound HealingSingle Cell MigrationActin-rich LamellaeActin CableEpithelial To Mesenchymal TransitionGene ExpressionMutantTransgenicIn Vitro Model

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