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Journal
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Immunology and Infection
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对白细胞从流程的分析招聘基质细胞的影响
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
Please note that all translations are automatically generated.
Click here for the English version.
对白细胞从流程的分析招聘基质细胞的影响
DOI:
10.3791/52480-v
•
11:30 min
•
January 07, 2015
•
Hafsa Munir
2
,
G. Ed Rainger
2
,
Gerard B. Nash
2
,
Helen McGettrick
3
1
School of Clinical and Experimental Medicine
,
University of Birmingham
,
2
College of Medical and Dental Sciences
,
University of Birmingham
,
3
School of Immunity and Infection
,
University of Birmingham
Chapters
00:05
Title
01:28
Assembling the Flow System
04:44
Setting Up Microslides and the Parallel Plate Flow Chamber Incorporating Filters for Flow
06:59
Perfusion of Leukocytes Over Endothelial Cells
07:55
Recording Neutrophil Capture and Behavior
09:47
Results: Evaluation of Neutrophil Recruitment to Endothelial Cells Cultured Alone or with MSC
11:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
发炎的内皮从流动招募白细胞的能力是由间充质基质细胞调节。我们描述两个
体外
结合原代人细胞,它可以用于从流动评估中性粒细胞募集和检查的作用是间充质基质细胞在调节这个过程中发挥的模型。
Tags
Endothelial Cells
Leukocyte Recruitment
Neutrophil Adhesion
Mesenchymal Stem Cells
In Vitro Vascular Model
Flow-based Adhesion Assay
TNF-alpha
Inflammatory Regulation
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