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Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
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Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
Chapters
- 00:05Title
- 01:28Assembling the Flow System
- 04:44Setting Up Microslides and the Parallel Plate Flow Chamber Incorporating Filters for Flow
- 06:59Perfusion of Leukocytes Over Endothelial Cells
- 07:55Recording Neutrophil Capture and Behavior
- 09:47Results: Evaluation of Neutrophil Recruitment to Endothelial Cells Cultured Alone or with MSC
- 11:06Conclusion
The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.
