The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.
Munir, H., Rainger, G. E., Nash, G. B., McGettrick, H. Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow. J. Vis. Exp. (95), e52480, doi:10.3791/52480 (2015).