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Real-time beeldvorming van Axonale Vervoer van Quantum Dot-label BDNF in primaire neuronen
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Neuroscience
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JoVE Journal Neuroscience
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
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Real-time beeldvorming van Axonale Vervoer van Quantum Dot-label BDNF in primaire neuronen

Nederlands

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14,914 Views

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10:53 min

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September 15, 2014

DOI:

10.3791/51899-v

DOI:
10.3791/51899-v

•

10:53 min
September 15, 2014

•

1 Views
, , , , ,
1Department of Neurosciences,University of California, San Diego, 2School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University, 3Department of Anesthesiology,University of California, San Diego, 4VA San Diego Healthcare System

Summary

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Axonale transport van BDNF, een neurotrofe factor, is van cruciaal belang voor de overleving en functie van verschillende neuronale populaties. Sommige degeneratieve stoornissen worden gekenmerkt door verstoring van axonale structuur en functie. We toonden de technieken die gebruikt worden om live handel in QD-BDNF in microfluïdische kamers met zowel primaire neuronen te onderzoeken.

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Cite this Article

Zhao, X., Zhou, Y., Weissmiller, A. M., Pearn, M. L., Mobley, W. C., Wu, C. Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons. J. Vis. Exp. (91), e51899, doi:10.3791/51899 (2014).

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