Rivista
/
Neuroscienze
/
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

DOI:
10.3791/51899-v

•

10:53 min

•

September 15, 2014

•

, , , , ,
1Department of Neurosciences,University of California, San Diego, 2School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University, 3Department of Anesthesiology,University of California, San Diego, 4VA San Diego Healthcare System

Capitoli

  • 00:05Titolo
  • 01:32Preparation of Microfluidic Chambers
  • 03:26Dissection of Neuronal Culture and Plating on Chambers
  • 05:41Axonal Transport of Quantum Dot-labeled Brain-derived Neurotrophic Factor (QD-BDNF)
  • 07:43Results: Live Imaging of Axonal Transport of QD-BDNF in Hippocampal Neurons
  • 10:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.

Tags

Axonal TransportBDNFQuantum DotNeurodegenerative DiseaseMicrofluidic DeviceRetrograde TransportAxonal Function

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Mechanical Manipulation of Neurons to Control Axonal Development

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

A Protocol for the Administration of Real-Time fMRI Neurofeedback Training

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Read Article