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Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

DOI:
10.3791/51899-v

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10:53 min

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September 15, 2014

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, , , , ,
1Department of Neurosciences,University of California, San Diego, 2School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University, 3Department of Anesthesiology,University of California, San Diego, 4VA San Diego Healthcare System

Chapters

  • 00:05Title
  • 01:32Preparation of Microfluidic Chambers
  • 03:26Dissection of Neuronal Culture and Plating on Chambers
  • 05:41Axonal Transport of Quantum Dot-labeled Brain-derived Neurotrophic Factor (QD-BDNF)
  • 07:43Results: Live Imaging of Axonal Transport of QD-BDNF in Hippocampal Neurons
  • 10:27Conclusion

Summary

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Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.

Tags

Axonal TransportBDNFQuantum DotNeurodegenerative DiseaseMicrofluidic DeviceRetrograde TransportAxonal Function

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