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Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
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JoVE Journal Neurowissenschaften
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

14,914 Views

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10:53 min

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September 15, 2014

DOI:

10.3791/51899-v

DOI:
10.3791/51899-v

•

10:53 min
September 15, 2014

•

1 Views
, , , , ,
1Department of Neurosciences,University of California, San Diego, 2School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University, 3Department of Anesthesiology,University of California, San Diego, 4VA San Diego Healthcare System

Summary

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Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.

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Zhao, X., Zhou, Y., Weissmiller, A. M., Pearn, M. L., Mobley, W. C., Wu, C. Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons. J. Vis. Exp. (91), e51899, doi:10.3791/51899 (2014).

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