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Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders
JoVE Journal
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JoVE Journal Nörobilim
Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

DOI:
10.3791/51853-v

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08:33 min

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December 04, 2014

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, , , , , , , , ,
1Psikiyatri Bölümü,Johns Hopkins Üniversitesi, 2Department of Psychiatry and Behavioral Sciences,Howard University, 3Department of Otolaryngology-Head and Neck Surgery,Johns Hopkins Üniversitesi, 4Psikiyatri Bölümü,Sheppard Pratt Hospital, 5Psikiyatri Bölümü,Indiana Üniversitesi

Bölümler

  • 00:05Başlık
  • 01:49Laser-Capture Microdissection
  • 03:48Isolation of Total RNA and cDNA Synthesis
  • 05:50Real Time PCR Quantification
  • 07:14Reults: GSK-3β Expression in OE Neurons
  • 07:53Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

In this study, a novel platform to investigate intraneuronal molecular signatures of treatment response in bipolar disorder (BD) was developed and validated. Olfactory epithelium from BD patients was obtained through nasal biopsies. Then laser-capture microdissection was combined with Real Time RT PCR to investigate the molecular signature of lithium response in BD.

Etiketler

Olfactory NeuronsNasal BiopsyLaser-capture MicrodissectionBipolar DisorderLithium CarbonateWnt SignalingNeuropsychiatric DisordersOlfactory EpitheliumTreatment Response

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